Ceratopteris: A Model Plant for the 90s February 1992 113 ... dent that the fern Ceratopteris richardii provides remarkable opportunities for developmental, physiological, genetic, ... Ceratopteris antheridiogen had appeared gibberellin-like as well because when However, except for spore germination (Cooke et al. Two models describing how antheridiogen may influence the activity states of these genes and the sex of the gametophyte are discussed. Cheruiyot DJ, Schwartz BW. Int J Plant Sci 156:367-373. View Article Google Scholar 14. Antheridiogen is a chemical secreted by fern gametophytes that has "been shown to influence production of male gametangia and thus mating systems in a ... More info. The pheromone antheridiogen promoted the production of males in gametophyte populations of the fern Ceratopteris richardii. This lab uses the fern Ceratopteris richardii (C-Fern), a model system for teaching and research that is particularly useful for illustrating alternation of generations. Ceratopteris The fern Ceratopteris richardii produces only one type of spore, yet each can develop as a male or a hermaphrodite gametophyte. Hieron. Abstract. Ceratopteris richardii gametophytes exhibit gender‐dependent differences in relative carbon and nitrogen content, and ESD in certain nutrient environments. antheridiogen indicate the involvement of the GAbiosyn-thetic pathway or other similar pathway in the synthesis of the Ceratopteris antheridiogen. The her1 mutation, which is one of many mutations that affect sexual differentiation in C-Fern, was induced by X-irradiation of spores.The effects of this mutation can be seen clearly in populations of gametophytes. BMC Res Notes DOI 10.1186/s13104-015-1193-x RESEARCH ARTICLE Transient and stable transformation of Ceratopteris richardii gametophytes Linh T Bui1, Angela R Cordle2, Erin E Irish2 and Chi‑Lien Cheng2* Abstract Background: Ferns, being vascular yet seedless, present unparalleled opportunities to investigate important ques‑ ?In the homosporous fern Ceratopteris richardii, sex is not determined chromosomally. The species-specific chemical messenger, antheridiogen ACe, mediates the differentiation of male gametophytes in the fern Ceratopteris . 1995). Spores were axenically cultured on half‐strength basal salt medium (Gamborg’s B‐5 BSM: G‐5768, Sigma Chemical Co., St Louis, MO) in Petri dishes sealed with parafilm at 25 °C with constant light (160 µmol photons m –2 s –1 ) in a walk‐in growth chamber. The determinant of sex is the pheromone antheridiogen, which is secreted by the hermaphrodite and promotes the development of males from sexually undertermined gametophytes. Gametophytes of the fern Ceratopteris are either male or hermaphroditic. Stable Transformation of Ferns Using Spores as Targets: Pteris vittata and Ceratopteris thalictroides1[W][OPEN] B. Muthukumar , B. Joyce , M. Elless , C. Stewart Plant Physiology In this work the effect of photomorphogenically active light on antheridiogen-induced male development was examined for gametophytes of Ceratopteris richardiiCeratopteris richardii Conversion of male gametophytes to hermaphrodites in the fern Ceratopteris richardii. ACKNOWLEDGEMENTS . The percentage of males increases with increasing density of gametophytes, presumably due to the cumulative effect of antheridiogen from multiple … Wild-type spores germinating in the absence of the pheromone will develop into hermaphroditic gametophytes, whereas spores continuously exposed to the pheromone will develop as males. Here we use RNA-seq to de novo assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. For different genetic strains, characteristic frequencies of sexual gametophytes primarily depend upon the relative sensitivity of gametophytes to antheridiogen. Recent analyses of sex determination mutants have defined a hypothetical network of … Ceratopteris richardii . Bui et al. Cloning of MADS Genes from Ceratopteris richardii. Ceratopteris constitute a group of aquatic ferns that possess both tracheids and vessels in the conducting tissues (Carlquist and Schneider, 2000).The evolutionary and functional significance of the presence of vessels in an aquatic fern with maximum availability of water still remains unsolved. C. richardii spores, in the absence of the hormone antheridiogen, will develop into hermaphrodite gametophytes, containing both egg-producing archegonia and sperm-producing antheridia [2, 6]. Taxonomic treatments by Robert M. Lloyd (1974, 1993) recognized four species: C. thalictroides, C. richardii, C. pteridoides and C. cornuta. C. richardii was previously regarded as being part of the species Ceratopteris thalictroides. Rather, hermaphroditic gametophytes produce a hormone called antheridiogen, which induces maleness in undifferentiated gametophytes. Their sex is epigenetically determined by the pheromone antheridiogen, which is secreted by the hermaphrodite and induces male and represses female development in other young, sexually undetermined gametophytes. Michael T. Ganger, Julia A. Girouard, Hannah M. Smith, Beth A. Bahny, Sarah J. Ewing Antheridiogen and abscisic acid affect conversion and ANI1 expression in Ceratopteris richardii gametophytes, Botany 93, no.2 2 (Feb 2015): 109-116. 175-179 ISSN: In the last decade, Ceratopteris richardii has been used as a new model species in ferns, because of easy isolation of mutants and a rapid life cycle (Chasen 1992, Hickok et al. Ceratopteris richardii (Hn‐n) (Scott and Hickok, 1987) spores were used in this study. Antheridiogen (ACE) is a pheromone that is required for the development of male gametophytes in the homosporous fern Ceratopteris richardii . Regional Science Consortium 6th Annual Research Symposium. The species-specific chemical messenger, antheridiogen, mediates the differentiation of male gametophytes in the fern Ceratopteris richardii Brongn. Populations of wild type C-Fern gametophytes show two sexual types, males and hermaphrodites. 1987), no phytochrome-mediated responses in C. richardii have been reported. —In the homosporous fern Ceratopteris richardii, sex is not determined chromosomally. We will also attempt to identify the genes in the fern Pteris vittata that make it able to hyperaccumulate and tolerate high levels of arsenic. Evidence for a Gibberellin Biosynthetic Origin of Ceratopteris Antheridiogen Evidence for a Gibberellin Biosynthetic Origin of Ceratopteris Antheridiogen Thomas R. Warne and Leslie G. Hickok 1989-02-01 00:00:00 ABSTRACT The species-specific chemical messenger, antheridiogen Ac*, mediates the differentiation of male gametophytes in the fern Ceratopteris. The sexuality of homosporous fern gametophytes is usually determined by antheridiogen, a pheromone that promotes maleness. The fern Ceratopteris richardii produces only one type of spore, yet each can develop as a male or a hermaphrodite gametophyte. The sex of the gametophyte of the homosporous fern Ceratopteris richardii (Brongn.) to antheridiogen, the primary sex-determining signal. Cooke TJ, LG Hickok, M Sugai 1995 Ceratopteris richardii as a model system for studying plant photobiology. The fern Ceratopteris richardii is an important model for studies of sex determination and gamete differentiation in homosporous plants. In Ceratopteris, the absence Antheridiogen C 4 . We will identify signaling molecules and genes in the fern Ceratopteris richardii that are necessary for sex determination and differentiation. Indian J Exp Biol 17:1403-1405. Antheridiogen concentration and spore size predict gametophyte size in Ceratopteris richardii Author: Ganger, Mike, Sturey, Tiffany Source: Botany 2012 v.90 no.3 pp. During spore germination, the first spores to germinate are hermaphrodites, which will secrete antheridiogen … This thesis means a lot to me because had it not been for my class Warne TR, Hickok LG. Control of Sexual Development in Gametophytes of Ceratopteris richardii: Antheridiogen and Abscisic Acid. The determinant of sex is the pheromone antheridiogen, which is secreted by the hermaphrodite and promotes the develop- … Candidate MADS-like Ceratopteris cDNA clones obtained by PCR using a MADS box-specific degenerate primer and an adapter primer were sorted into five distinct groups depending on their digestion patterns with … Before I begin, I’d like to explain that this thesis is something that marks the culmination of my undergraduate career—an undergraduate career that started off terribly and improved tremendously up until the end. Sturey T and Ganger M (2010) Size determinants of Ceratopteris richardii and their potential influence on gender. In contrast, this maleless mutant does not contain male gametophytes in populations. Botanical Gazette. H APLOID gametophytes of many homosporous ferns, including Ceratopteris richardii… This lab does not simply present the stages of the life cycle; it also uses knowledge of alternation of generations as a starting point for characterizing the her1 mutation that affects gametophyte sexual development. 1991;152(2):148–53. Erie, PA; Sturey T and Ganger M (2010) The effect of antheridiogen concentration on conversion and size of male gametophytes of Ceratopteris richardii. Subtractive hybridization of cDNAs isolated from ACE-treated and non-ACE-treated gametophytes was used to isolate genes that are induced by the pheromone. Induction by Antheridiogen in Ceratopteris richardii Asya Ayrapetov* and Michael T. Ganger Department of Biology, Gannon University, 109 University Square, Erie, PA 16541-0001 Abstract. is determined by the pheromone antheridiogen (ACE). Cheema HK 1979 Morphogenetic studieson the induction of aposporyin Ceratopteris pteridoides (Hook.) GENETIC ANALYSIS OF ANTHERIDIOGEN SENSITIVITY IN CERATOPTERIS RICHARDII1 RODNEY J. SCOTT AND LESLIE G. HICKOK Department of Botany, University of Tennessee, Knoxville, Tennessee 37996-1 100 ABSTRACT The pheromone antheridiogen promoted the production of males in gametophyte populations of the fern Ceratopteris richardii. Spores of the fern Ceratopteris richardii can develop as either males or hermaphrodites; the difference in sex is determined by the pheromone antheridiogen secreted by the hermaphrodite. While some treatments have placed Ceratopteris in its own monotypic family, Parkeriaceae, others have included it as a subfamily or tribe within the Pteridaceae. It is one of several genera of ferns adapted to an aquatic existence. in aseptic culture. Thus, Ceratopteris (Parkeri-aceae) andthe Schizeaceae maypossess similar biosynthetic pathwaysforantheridiogen productionthat share features in commonwith GAbiosynthesis. 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